PBJ：浙大张国平丨HvAKT2和HvHAK1通过增强叶肉H+稳态提升耐旱能力

期刊：Plant Biotechnology Journal
主题：HvAKT2和HvHAK1通过增强叶肉H+稳态提升耐旱能力

标题：HvAKT2 and HvHAK1 Confer Drought Tolerance in Barley through Enhanced Leaf Mesophyll H+ Homeostasis

影响因子：6.840
检测指标：K+、H+、Ca2+流速

检测样品：大麦叶肉细胞

K+、H+、Ca2+流实验处理方法：

1）20% PEG瞬时处理大麦叶片
制备叶肉组织，测试液中平衡2h后，用20％ PEG瞬时处理
2）20%PEG预处理
制备叶肉组织，用20% PEG处理0、1、12、24h
K+、H+、Ca2+流实验测试液成份：
0.1 mMCaCl2，0.5 mM KCl，pH 5.8

作者：浙江大学作物科学研究所张国平、邬飞波、陈仲华、冯雪

Plant K+ uptake typically consists low゛ffinity  mechanisms mediated by Shaker K+ channels (AKT/KAT/KC) and high゛ffinity  mechanisms regulated by HAK/KUP/KT transporters, which are extensively  studied. However, the evolutionary and genetic roles of both K+ uptake  mechanisms for drought tolerance are not fully explored in crops adapted  to dryland agriculture.

Here, we employed evolutionary  bioinformatics, biotechnological and electrophysiological approaches to  determine the role of two important K+ transporters HvAKT2 and HvHAK1 in  drought tolerance in barley. HvAKT2 and HvHAK1 were cloned and  functionally characterized using barley stripe mosaic virus﹊nduced gene  silencing (BSMV￢IGS) in droughtPBJ：浙大张国平丨HvAKT2和HvHAK1通过增强叶肉H+稳态提升耐旱能力