NMT历史上的今天丨Plant Soil 植物Pb、Cu重金属胁迫 文章发表

NMT是基因功能的活体检测技术，已被31位诺贝尔奖得主所在单位，及北大、清华、中科院使用。​

2016年11月13日，中科院烟台海岸带研究所骆永明、李连祯利用NMT在Plant and Soil 上发表了标题为Determining  the fluxes of ions (Pb2+, Cu2+ and Cd2+) at the root surface of wetland  plants using the scanning ion-selective electrode technique的研究成果。

期刊：Plant and Soil

主题：使用扫描离子选择电极技术确定湿地植物根表面离子（Pb2+，Cu2+和Cd2+）的通量

标题：Determining the fluxes of ions (Pb2+, Cu2+ and  Cd2+) at the root surface of wetland plants using the scanning  ion-selective electrode technique

影响因子：3.410

检测指标：Pb2+、Cu2+、Cd2+流速

作者：中科院烟台海岸带研究所骆永明、李连祯

英文摘要

Measuring specific ion fluxes from different regions  of the root under practical physiological conditions is crucial for  understanding metal uptake mechanisms by plants.

We developed and tested a neutral carrier-based  liquid-membrane Pb2+ and Cu2+ ion selective microelectrode (ISME) to  investigate ion-transport processes along the roots of three common  wetland plant species.

The Pb2+ and Cu2+ ISME exhibited a Nernstian response  with Pb2+ and Cu2+ activities as low as 1.0 nM and 1.0 μM in deionized  water and simulated soil solution, respectively. Phragmites australis  had a region of Cu2+ release for approximately the first 200 μm, while  it exhibited Pb2+ and Cd2+ outward net flux up to the first 500 μm.  Although in older sections of the root of Phragmites australis there  were areas of influx of Cu2+, Pb2+ and Cd2+, the overall influx was much  smaller than that of Typha latifolia or Canna indica. Such a reduced  uptake and/or an increased efflux of metal ions across the root-cell  plasma-membrane might explain the higher resistance of Phragmites  australis to metals, at least in part.

The Pb2+ and Cu2+ ISMEs are shown to permit detailed  investigation of heavy-metal ion transport in plant roots, especially  for plants used for phytoremediation.

中文摘要（谷歌机翻）

Fig. 3 Measurement of fluxes (outward positive) of  Cd2+,Pb2+ and Cu2+ (mean ± standard error) across the root tips of three  common wetland plant species, Typha latifolia (a) Canna indic (b) and  Phragmites australis (c), using Cd2+,Pb2+ and Cu2+ ion selective  microelectrodes and the scanning ion-selective electrode higher metal  influx in the root regions, especially in the meristematic and  elongation zone (Fig. 3).