NEW PHYTOL 北京林业大学丨菌根通过维持植物NO3-的吸收以应对盐胁迫

期刊：New Phytologist

主题：菌根通过维持植物NO3-的吸收以应对盐胁迫

标题：Amelioration of nitrate uptake under salt stress by ectomycorrhiza with and without a Hartig net

影响因子：7.433

检测指标：NO3-流速

检测部位：杨树根（距离根尖300-400μm）

NO3-流速流实验处理方法：

小植株杨树在无/有菌株（MAJ和NAU）的情况下培养30d，之后在0或100mM NaCl处理24h/7d

NO3-流速流实验测试液成份：
NO3- measuring solution: 0.1 mM KNO3, 0.1 mM KCl, 0.1 mM CaCl2, pH 5.3 was adjusted with KOH and HCl.
H+ measuring solution: 0.1 mM NaCl, 0.1 mM MgCl2, 0.1 mM CaCl2 and 0.5 mM KCl, pH 5.3 was adjusted with KOH and HCl.

作者：北京林业大学陈少良、撒刚、邓晨

英文摘要

Salt stress is an important environmental cue  impeding poplar nitrogen nutrition. Here, we characterized the impact of  salinity on protonヾriven nitrate fluxes in ectomycorrhizal roots and  the importance of a Hartig net for nitrate uptake.

We employed  two Paxillus involutus strains for root colonization: MAJ, which forms  typical ectomycorrhizal structures (mantle and Hartig net), and NAU,  colonizing roots with a thin, loose hyphal sheath. Fungusヽolonized and  noncolonized Populus × canescens were exposed to sodium chloride and  used to measure root surface pH, nitrate (NO3−) flux and transcription  of NO3− transporters (NRTs; PcNRT1.1, NEW PHYTOL 北京林业大学丨菌根通过维持植物NO3-的吸收以应对盐胁迫