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NEW PHYTOL 北京林业大学丨菌根通过维持植物NO3-的吸收以应对盐胁迫

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NEW PHYTOL 北京林业大学丨菌根通过维持植物NO3-的吸收以应对盐胁迫

期刊:New Phytologist
主题:菌根通过维持植物NO3-的吸收以应对盐胁迫
标题:Amelioration of nitrate uptake under salt stress by ectomycorrhiza with and without a Hartig net
影响因子:7.433
检测指标:NO3-流速
检测部位:杨树根(距离根尖300-400μm)
NO3-流速流实验处理方法:
小植株杨树在无/有菌株(MAJ和NAU)的情况下培养30d,之后在0或100mM NaCl处理24h/7d
NO3-流速流实验测试液成份:
NO3- measuring solution: 0.1 mM KNO3, 0.1 mM KCl, 0.1 mM CaCl2, pH 5.3 was adjusted with KOH and HCl.
H+ measuring solution: 0.1 mM NaCl, 0.1 mM MgCl2, 0.1 mM CaCl2 and 0.5 mM KCl, pH 5.3 was adjusted with KOH and HCl.
作者:北京林业大学陈少良、撒刚、邓晨
英文摘要

Salt stress is an important environmental cue  impeding poplar nitrogen nutrition. Here, we characterized the impact of  salinity on protonヾriven nitrate fluxes in ectomycorrhizal roots and  the importance of a Hartig net for nitrate uptake.

We employed  two Paxillus involutus strains for root colonization: MAJ, which forms  typical ectomycorrhizal structures (mantle and Hartig net), and NAU,  colonizing roots with a thin, loose hyphal sheath. Fungusヽolonized and  noncolonized Populus × canescens were exposed to sodium chloride and  used to measure root surface pH, nitrate (NO3−) flux and transcription  of NO3− transporters (NRTs; PcNRT1.1, NEW PHYTOL 北京林业大学丨菌根通过维持植物NO3-的吸收以应对盐胁迫
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