JIPB：种康院士 | OsCIPK7改变激酶活性调控水稻耐寒机制（钙信号）

期刊：Journalof integrative plant biology（JIPB）

主题：OsCIPK7改变激酶活性调控水稻耐寒机制（钙信号)

标题：OsCIPK7point-mutation leads to conformation and kinase-activity change for sensingcold response

影响因子：3.129

检测指标：Ca2+

作者：中科院植物所 种康

Calcineurin B-like interacting proteinkinases (CIPKs) play important roles via environmental stress. However, less isknown how to sense the stress in molecular structure conformation level.

Here, an OsCIPK7 mutant via TILLINGprocedure with a point mutation in the kinase domain showed increased chillingtolerance, which could be potentially used in the molecular breeding.

We found that this point mutation ofOsCIPK7 led to a conformational change in the activation loop of the kinasedomain, subsequently with an increase of protein kinase activity, thusconferred an increased tolerance to chilling stress.

中文摘要（谷歌机翻）

NMT measurements show dynamic change of extracellular Ca2+ influx in live roots of various genetic backgrounds responding to cold shock. The blue background represents the duration of cold treatment. The number was the cold treatment time on the abscissa. The minus numbers were the time before the treatment.