NMT作为生命科学底层核心技术，是建立活体创新科研平台的必备技术。2005年~2020年，NMT已扎根中国15年。2020年，中国NMT销往瑞士苏黎世大学，正式打开欧洲市场。
研究使用平台：NMT植物耐盐创新科研平台 期刊：Molecular Plant 主题：Na+流为SOS调控植物耐盐新机制研究提供证据 标题：ESCRT-I component VPS23A sustains salt tolerance by strengtheningthe SOS module in Arabidopsis 影响因子：12.084 检测指标：Na+流速 检测样品：拟南芥根部分生区 Na+流实验处理方法： 12日龄的拟南芥在150 mM NaCl处理5h Na+流实验测试液成份： 0.1 mM CaCl2, 0.1 mM KCl, 0.5 mMNaCl, and 0.3 mM MES，pH5.8 作者：谢旗、郭岩、于菲菲、娄丽娟
中文摘要 含钠转运蛋白SOS1和调节蛋白SOS2和SOS3的盐分过度敏感（SOS）信号模块，众所周知是帮助植物抵抗盐分积累的中央盐分排泄系统。
在这里我们报告说，VPS23A是运输所需的内体分选复合物（ESCRT）的组成部分，在SOS模块赋予植物耐盐性的功能中起着至关重要的作用。VPS23A增强了SOS2 / SOS3复合体的相互作用。
在存在盐胁迫的情况下，VPS23A积极调节SOS2向质膜的重新分布过程，然后激活SOS1的反转运蛋白活性以减少植物细胞中的Na+积累。遗传证据表明，通过SOS2和SOS3的过表达实现的耐盐性取决于VPS23A。两者合计，我们的结果表明，VPS23A是SOS模块的关键调节因子，可影响SOS2在细胞膜上的亚细胞定位。
此外，膜结合的SOS2赋予拟南芥幼苗较强的耐盐性，揭示了SOS2分选对细胞膜发挥作用的重要性。
(D and E)Net Na+ fluxes in root tips.Non-invasive Micro-test Technology (NMT) was used for Na+ flux measurement invivo. 10-day-old seedlings cultured in liquid ½MS medium were treated with 0(D, upper panel) or 150 mM NaCl (D, lower panel) for 5 h, and then thecontinuous transient Na+ fluxes were recorded for about 6 min. Each point isthe mean of four individual plants (D). Quantitative analysis of the means ofnet Na+ fluxes within the continuous period of 0–6 min as shown in (E).Thevalues are means ± SD of three independent repeats. *P value <0.05.
英文摘要 Salt-Overly-Sensitive (SOS) signalingmodule comprising the sodium-transport protein SOS1 and the regulatory proteinsSOS2 and SOS3, is well known as the central salt excretion system that helpsplants against salt accumulation.
Here we report that VPS23A, a component ofthe endosomal sorting complex required for transport (ESCRT), plays anessential role in the function of the SOS module to confer plant salttolerance. VPS23A enhances the interaction of the SOS2/SOS3 complex.
In the presence of salt stress, VPS23Apositively regulates the process of re-distribution of SOS2 to the plasmamembrane and then activates the antiporter activity of SOS1 to reduce Na+accumulation in plant cells. Genetic evidence demonstrated that salt tolerance achieved by the overexpression of SOS2and SOS3 depended on VPS23A. Taken together, our results identified that VPS23Ais a crucial regulator of the SOS module that affects the subcellularlocalization of SOS2 to the cell membrane.
Moreover, the strong salt tolerance of Arabidopsisseedlings conferred by a membrane-bound SOS2 revealed the significance of theaction of SOS2 sorting to the cell membrane in achieving its function.
关键词：非损伤微测技术，Na+流速，盐胁迫
MP谢旗郭岩：Na+流为SOS调控植物耐盐新机制研究提供证据 | NMT植物耐盐创新科研平台 | 
楼主
提升卡
置顶卡
沉默卡
喧嚣卡
变色卡
千斤顶
照妖镜