研究使用平台：NMT微藻创新科研平台
期刊：Nature Climate Change
主题：NMT钙流为气候变化导致冰藻运动能力下降提供信号调节证据
标题：Decreased motility of flagellated microalgae
long-term acclimated to CO2-inducedacidified waters
影响因子：21.72
检测指标：Ca2+流速
检测样品：极地冰藻
Ca2+流实验处理方法：
极地冰藻在280、400、700、1,000、1,500和2,000 ppm的CO2浓度环境下，传代培养5年
Ca2+流实验测试液成份：
0.2 mM CaCl2, 360 mM NaCl, 2.0mM NaHCO3, 8.0 mM KCl, 0.1 mM Na2SO4, 0.05 mM H3BO3,0.5 mM NH4NO3, 2.0 mM Tris
作者：中国水产科学研究院黄海水产研究所叶乃好、王依涛
中文摘要（谷歌机翻）
运动在藻类的生存和繁殖中起着至关重要的作用，对水生生态系统的稳定性具有重要意义。但是，尚不清楚二氧化碳浓度升高对海洋，咸淡水和淡水藻类运动的影响。
在这里，我们使用实验室的微尺度和中尺度尺度的实验表明，三种典型的浮游植物物种随着二氧化碳的增加而运动性降低。极地海洋Microglena sp.，欧洲盐藻杜氏盐藻和淡水莱茵衣藻在不同二氧化碳浓度下生长了5年。长期适应的Microglena sp。在所有处理中均显示出显着降低的光反应，并且有憎光反应影响细胞内钙浓度。
调节鞭毛运动的基因被显着下调（P<0.05），同时鞭毛脱落的基因表达也显着增加（P <0.05）。D. salina和C. reinhardtii表现出相似的结果，表明活力变化在鞭毛物种中很常见。
由于鞭毛的结构和弯曲机制从单细胞生物到脊椎动物都是保守的，这些结果表明增加的地表水二氧化碳浓度可能影响从藻类到鱼类的鞭毛细胞。
The meanflux of Ca2+ under different pCO2 I scenarios. Mean ±s.d. values per experimental assay are given (n = 3). Upper, Ca2+efflux under positive phototaxis. Lower, Ca2+ influx under negativephototaxis. ‘−’ on the vertical scale means Ca2+ entry. LC4,flagellar outer dynein arm light chain 4; DC3, outer dynein arm docking complexprotein 3; IC138, arm dynein; RSP, radial spoke protein; PF20, a protein of thecentral pair apparatus; PKA, cAMP-dependent protein kinase; PP2A, proteinphosphatase 2A; CK1, casein kinase DIP13/NA14, deflagellation inducible protein; DNAAF3/PF22, axonemaldynein assembly factor. Mean ± s.d. values per experimental assay are given (n= 3). Different letters (a–c) in panelindicate significant differences (P < 0.05) among treatments.
英文摘要
Motilityplays a critical role in algal survival and reproduction, with implications foraquatic ecosystem stability. However, the effect of elevated CO2 onmarine, brackish and freshwater algal motility is unclear.
Here weshow, using laboratory microscale and field mesoscale experiments, that threetypical phytoplankton species had decreased motility with increased CO2.Polar marine Microglena sp., euryhaline Dunaliella salina and freshwaterChlamydomonas reinhardtii were grown under different CO2 concentrationsfor 5 years. Long-term acclimated Microglena sp. showed substantially decreasedphoto-responses in all treatments, with a photophobic reaction affectingintracellular calcium concentration.
Genesregulating flagellar movement were significantly downregulated (P < 0.05),alongside a significant increase in gene expression for flagellar shedding (P< 0.05). D. salina and C. reinhardtii showed similar results, suggestingthat motility changes are common across flagellated species.
As theflagella structure and bending mechanism are conserved from unicellularorganisms to vertebrates, these results suggest that increasing surface waterCO2 concentrations may affect flagellated cells from algae to fish.
关键词：非损伤微测技术，Ca2+流速，极地冰藻
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