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NMT作为生命科学底层核心技术，是建立活体创新科研平台的必备技术。2005年~2020年，NMT已扎根中国15年。2020年，中国NMT销往瑞士苏黎世大学，正式打开欧洲市场。
研究使用平台：NMT植物重金属创新科研平台 期刊：Journal of HazardousMaterials 主题：NMT发现杨树雌雄株根系吸Cd速率差异揭示其不同耐Cd策略 标题：Are males andfemales of Populus cathayana differentially sensitive to Cd stress 影响因子：7.65 检测指标：Cd2+流速 检测样品：杨树 Cd2+流实验处理方法： 雌雄杨树幼苗在0或50μM CdCl2中处理120天 Cd2+流实验测试液成份： 0.05 mM CdSO4、0.25 mM NaCl，0.05 mM KCl，0.1 mMNa2SO4，pH 6.0 作者：杭州师范大学李春阳、刘秒
中文摘要（谷歌机翻） 这项研究阐明了白杨在雄性和雌性中镉吸收，转运和排毒的机理，并揭示了一种雌雄异株的植物应对镉污染的新策略。
雌性显示出高水平的Cd吸收和从根到茎的移位，而雄性则显示出根中大量Cd积累，提高的抗氧化能力以及有效的细胞和树皮Cd螯合。
我们的研究还发现，镉主要位于雄性根和叶的表皮和皮质组织中，而在雌性中，根和叶的维管组织以及叶肉中存在更多的镉。另外，雄性中硫（S）和磷（P）的分布与镉的分布非常相似，但雌性的相关性较弱。
扫描电子显微镜和能谱分析表明，雄性中组织镉的含量与磷和磷含量呈正相关，而雌性中磷含量和硫含量却没有正相关（S和Cd之间的相关性较弱）。转录数据表明，Cd胁迫促进了雌性与Cd吸收和转运相关的基因的上调，而雄性则促进了与细胞壁生物合成，金属耐受性和次级代谢有关的基因的上调。
我们的研究结果表明，与雌性相比，对镉胁迫的协调生理，微观结构和转录应答赋予雄性更高的镉耐受性，并且为对镉胁迫的性差异应答的潜在机制提供了新见解。
Net Cd2+ fluxes in apical and mature zones of roots
英文摘要 Thisstudy clarifies the mechanisms of Cd uptake, translocation and detoxificationin Populus cathayana Rehder females and males, and reveals a novel strategy fordioecious plants to cope with Cd contamination.
Females exhibited a high degree of Cd uptake and root-to-shoot translocation,while males showed extensive Cd accumulation in roots, elevated antioxidativecapacity, and effective cellular and bark Cd sequestration.
Our study also found that Cd is largely located in epidermal and corticaltissues of male roots and leaves, while in females, more Cd was present invascular tissues of roots and leaves, as well as in leaf mesophyll. Inaddition, the distributions of sulphur (S) and phosphorus (P) were very similaras that of Cd in males, but the associations were weak in females.
Scanning electron microscopy and energy spectroscopy analyses suggested thatthe amounts of tissue Cd were positively correlated with P and S amounts inmales, but not in females (a weak correlation between S and Cd). Transcriptionaldata suggested that Cd stress promoted the upregulation of genes related to Cduptake and translocation in females, and that of genes related to cell wallbiosynthesis, metal tolerance and secondary metabolism in males.
Our results indicated that coordinated physiological, microstructural andtranscriptional responses to Cd stress endowed superior Cd tolerance in malescompared with females, and provided new insights into mechanisms underlyingsexually differential responses to Cd stress.
关键词：非损伤微测技术，Cd2+流速，杨树，植物重金属
JHM：NMT发现杨树雌雄株吸镉速率差异揭示其不同耐镉策略 | 
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