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MP谢旗郭岩:Na+流为SOS调控植物耐盐新机制研究提供证据 | NMT植物耐盐创新科研平台

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MP谢旗郭岩:Na+流为SOS调控植物耐盐新机制研究提供证据 | NMT植物耐盐创新科研平台

NMT作为生命科学底层核心技术,是建立活体创新科研平台的必备技术。2005~2020年,NMT已扎根中国15年。2020年,中国NMT销往瑞士苏黎世大学,正式打开欧洲市场。


研究使用平台:NMT植物耐盐创新科研平台
期刊:Molecular Plant
主题:Na+流为SOS调控植物耐盐新机制研究提供证据
标题:ESCRT-I component VPS23A sustains salt tolerance by strengtheningthe SOS module in Arabidopsis
影响因子:12.084
检测指标:Na+流速
检测样品:拟南芥根部分生区
Na+流实验处理方法:
12日龄的拟南芥在150 mM NaCl处理5h
Na+流实验测试液成份:
0.1 mM CaCl2, 0.1 mM KCl, 0.5 mMNaCl, and 0.3 mM MESpH5.8
作者:谢旗、郭岩、于菲菲、娄丽娟




中文摘要
含钠转运蛋白SOS1和调节蛋白SOS2SOS3的盐分过度敏感(SOS)信号模块,众所周知是帮助植物抵抗盐分积累的中央盐分排泄系统。


在这里我们报告说,VPS23A是运输所需的内体分选复合物(ESCRT)的组成部分,在SOS模块赋予植物耐盐性的功能中起着至关重要的作用。VPS23A增强了SOS2 / SOS3复合体的相互作用。


在存在盐胁迫的情况下,VPS23A积极调节SOS2向质膜的重新分布过程,然后激活SOS1的反转运蛋白活性以减少植物细胞中的Na+积累。遗传证据表明,通过SOS2SOS3的过表达实现的耐盐性取决于VPS23A。两者合计,我们的结果表明,VPS23ASOS模块的关键调节因子,可影响SOS2在细胞膜上的亚细胞定位。


此外,膜结合的SOS2赋予拟南芥幼苗较强的耐盐性,揭示了SOS2分选对细胞膜发挥作用的重要性。






(D and E)Net Na+ fluxes in root tips.Non-invasive Micro-test Technology (NMT) was used for Na+ flux measurement invivo. 10-day-old seedlings cultured in liquid ½MS medium were treated with 0(D, upper panel) or 150 mM NaCl (D, lower panel) for 5 h, and then thecontinuous transient Na+ fluxes were recorded for about 6 min. Each point isthe mean of four individual plants (D). Quantitative analysis of the means ofnet Na+ fluxes within the continuous period of 0–6 min as shown in (E).Thevalues are means ± SD of three independent repeats. *P value <0.05.


英文摘要
Salt-Overly-Sensitive (SOS) signalingmodule comprising the sodium-transport protein SOS1 and the regulatory proteinsSOS2 and SOS3, is well known as the central salt excretion system that helpsplants against salt accumulation.

Here we report that VPS23A, a component ofthe endosomal sorting complex required for transport (ESCRT), plays anessential role in the function of the SOS module to confer plant salttolerance. VPS23A enhances the interaction of the SOS2/SOS3 complex.

In the presence of salt stress, VPS23Apositively regulates the process of re-distribution of SOS2 to the plasmamembrane and then activates the antiporter activity of SOS1 to reduce Na+accumulation in plant cells. Genetic evidence demonstrated that salt tolerance achieved by the overexpression of SOS2and SOS3 depended on VPS23A. Taken together, our results identified that VPS23Ais a crucial regulator of the SOS module that affects the subcellularlocalization of SOS2 to the cell membrane.

Moreover, the strong salt tolerance of Arabidopsisseedlings conferred by a membrane-bound SOS2 revealed the significance of theaction of SOS2 sorting to the cell membrane in achieving its function.



关键词:非损伤微测技术,Na+流速,盐胁迫




MP谢旗郭岩:Na+流为SOS调控植物耐盐新机制研究提供证据 | NMT植物耐盐创新科研平台

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